In this paper we present results of a series of bandwidth estimation experiments conducted on a high-speed testbed at the San Diego Supercomputer Center and on OC-48 and GigE paths in real world networks. We test and compare publicly available bandwidth estimation tools: abing, pathchirp, pathload, and Spruce. We also tested Iperf which measures achievable TCP throughput. In the lab we used two different sources of known and reproducible cross-traffic in a fully controlled environment. In real world networks we had a complete knowledge of link capacities and had access to SNMP counters for independent cross-traffic verification. We compare the accuracy and other operational characteristics of the tools and analyze factors impacting their performance.